Transforming Nanofiber Membranes Into Complex 3D Shapes for Biomedical Applications
Transforming Nanofiber Membranes Into Complex 3D Shapes for Biomedical Applications. In the movie “Transformers,” cars morph into robots, jets or a variety of machinery. A similar concept inspired a group of researchers to combine gas foaming, which is a blend of chemicals that induces gas bubbling, and 3D molding technologies to quickly transform electrospun membranes into complex 3D shapes for biomedical applications.
In Applied Physics Reviews, from AIP Publishing, the group reports on its new approach that demonstrates significant improvements in speed and quality compared with other methods. The work is also the first successful demonstration of formation of 3D neural tissue constructs with an ordered structure through differentiation of human neural progenitor/stem cells on these transformed 3D nanofiber scaffolds.
“Electrospinning is a technology to produce nanofiber membranes,” said co-author Jingwei Xie, at the University of Nebraska Medical Center. “The physics principle behind it involves applying an electrical force to overcome the surface tension of a solution to elongate a solution jet into continuous and ultrafine fibers after solvent evaporation.”
“Our 3-D objects have the appropriate pore size and controlled fiber alignment for guiding and enhancing cell penetration to form new tissue,” Xie said. The group’s work is significant, because it can be done within an hour. Other methods can require up to 12 hours to complete the transformation process.
“Thanks to the ability to mimic the architecture of extracellular matrix, electrospun nanofibers show great potential in applications such as tissue engineering, regenerative medicine and tissue modeling,” said Xie. One of the group’s most intriguing findings is that after coating 3-D nanofiber objects with gelatin, they exhibit superelasticity and shape recovery.
“Gelatin-coated, cube-shaped scaffolds functionalized with polypyrrole coatings exhibited dynamic electrical conductivity during cyclical compression,” he said. They also demonstrated that cuboid-shaped nanofiber objects were effective for compressible hemorrhage in a pig liver injury model.